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Rapid cell-free characterization of multi-subunit CRISPR effectors and transposons. Wimmer F, Mougiakos I, Englert F, Beisel CL. Mol Cell. 2022 Mar 17;82(6):1210-1224.e6. doi: 10.1016/j.molcel.2022.01.026. Epub 2022 Feb 24.
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A TXTL-Based Assay to Rapidly Identify PAMs for CRISPR-Cas Systems with Multi-Protein Effector Complexes. Wimmer F, Englert F, Beisel CL. Methods Mol Biol. 2022;2433:391-411. doi: 10.1007/978-1-0716-1998-8_24.
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Noncanonical crRNAs derived from host transcripts enable multiplexable RNA detection by Cas9. Jiao C, Sharma S, Dugar G, Peeck NL, Bischler T, Wimmer F, Yu Y, Barquist L, Schoen C, Kurzai O, Sharma CM, Beisel CL. Science. 2021 May 28;372(6545):941-948. doi: 10.1126/science.abe7106. Epub 2021 Apr 27.
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An enhanced assay to characterize anti-CRISPR proteins using a cell-free transcription-translation system. Wandera KG, Collins SP, Wimmer F, Marshall R, Noireaux V, Beisel CL. Methods. 2019 May 21. pii: S1046-2023(19)30001-5. doi: 10.1016/j.ymeth.2019.05.014. [Epub ahead of print]
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CRISPR-Cas Systems and the Paradox of Self-Targeting Spacers. Wimmer F, Beisel CL. Front Microbiol. 2020 Jan 22;10:3078. doi: 10.3389/fmicb.2019.03078. eCollection 2019. Review.
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